Week 13. Semester 3. Sequencing trip to ASU

Thursday 12/03 we visited ASU to drop off samples for sequencing. The samples dropped were labeled mip 1 through 10. 

Utilizing a NanoDrop to quantify the mass of DNA in each tube, the mass for each sample was recorded in ng/mL.

First the NanoDrop was blanked with 1µL of sterile water. Only one blank was necessary for all the samples.

The apparatus was wiped with a KimWipe in between the blank and each sample.

1µL of each sample and positive control (purified from agarose gels) was used per sample.

Once the mass was recorded, the tubes were prepared as follows for sequencing:

Each sample had to contain at least 20ng per tube. The smallest mass recorded was ≈5ng/mL. Therefore, 4µL of each sample and controls were added to each labeled tube along with another volume of forward primer. Depending on the sample type, mip or 16s, 2µL primer was added.

Visiting ASU has been a very nice experience. The man running the lab, Scott is always very nice and I feel our short talks and this explanations of how sequencing works have been a meaningful part of my experience this semester.

ASU main

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