Week 3. Semester 3. PCR products analysis. Not a success

This Friday, September 18th, we analyzed the PCR products in an agarose gel under UV light in order to asses whether we were ready to continue analyzing field samples for the presence of Legionella. As you can see in the picture below, we were unsuccessful since there was a lot of shearing in the gels and we will be repeating the protocols on the same PCR products next Tuesday. Then next Friday we plan to repeat PCR in hopes we get better results and can move forward with the experiment. 

Analysis on Agarose Gel Electrophoresis (9-18-15)

1% Agarose gel with 1μL/1% SYBER®Green.

Loaded:

Molecular Weight Marker (2 μL).

positive control DNA (5 μL)

loading dye (2 μL)

2 μL water

Loaded 7 μL of control DNA sample and 2 μL of molecular weight marker.

Ran gel at 100 volts for 45-50 minutes in a 1x TAE Buffer solution (490mL dI water +10 mL 50xTAE)

Placed gel in gel doc to analyze banding.

16s control DNA gel

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
MWM	7-7-15		7-8-15		7-13-15	7-30-15

Mip control DNA gel

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
MWM		7-7-15	7-8-15		7-13-15	7-27-15