Week 4. Semester 3. Gel re-run and plans for today

This week we re-ran the gels for the previous unsuccessful PCR amplification of positive controls and got the same results. We used a freshly prepared buffer and new loading dye to make sure the problem was not in the gels. Today we will be working on re-running the PCR for the same positive controls with new primers in hopes we can get nice banding in the gels next week. Protocol:
Analysis on Agarose Gel Electrophoresis (9-22-15)

1% Agarose gel with 1μL/1% SYBER®Green.

Loaded:

Molecular Weight Marker (2 μL).

Positive control DNA (2 μL)

loading dye (2 μL)

3 μL water

Loaded the full 7 μL of control DNA mixture (PCR product, loading dye and water) and 2 μL of molecular weight marker.

Ran gel at 100 volts for 45-50 minutes in a 1x TAE Buffer solution (490mL dI water +10 mL 50xTAE)

Placed gel in gel doc to analyze banding.

16s 

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
MWM		7-7-15	7-8-15		7-13-15	7-30-15

mip

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
MWM		7-7-15	7-8-15		7-13-15	7-27-15