Week 5. Semester 3. Not good

Last Friday we re-ran a PCR for the stock positive controls and this Tuesday, September 29th, we ran a gel to analyze the banding. We were hoping the problem would be in the Primers we used last time and used brand newly aliquoted Primers but still got good bands. We pretty much had the same results are last time. There was a very small band at the appropriate distance for 16s (7/13/2015). Please refer to picture below.

Analysis on Agarose Gel Electrophoresis (9-29-15)

1% Agarose gel with 1μL/1% SYBER®Green.

Loaded:

Molecular Weight Marker (2 μL).

positive control DNA (2 μL)

loading dye (2 μL)

3 μL water

Loaded 7μL of control DNA sample and 2 μL of molecular weight marker.

Ran gel at 100 volts for 45-50 minutes in a 1x TAE Buffer solution (490mL dI water +10 mL 50xTAE)

Placed gel in gel doc to analyze banding.

 16s

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
	MWM		7-7-15		7-13-15	7-30-15